Tag Archives: minION

What is ‘real-time’ phylogenomics?

Over the past few years I’ve been developing research, which I collectively refer to as ‘real-time phylogenomics’ – and this is the name of our mini-site for MinION-based rapid identification-by-sequencing. Since our paper on this will hopefully be published soon, … Continue reading

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Some aspects of BLASTing long-read data

Quick note to explain some of the differences we’ve observed working with long-read data (MinION, PacBio) for sample ID via BLAST. I’ll publish a proper paper on this, but for now: Long reads aren’t just a bit longer than Illumina data, … Continue reading

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More MinION – the ‘1D rapid’ prep

My last MinION post described our first experiments with this really cool new technology. I mentioned then that their standard library prep was fairly involved, and we heard that the manufacturers, Oxford Nanopore, were working on a faster, simpler library prep. We … Continue reading

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Copying LOADS of files from a folder of LOADS *AND* LOADS more in OSX

Quick one this, as it’s a tricky problem I keep having to Google/SO. So I’m posting here for others but mainly myself too! Here’s the situation: you have a folder (with, ooh, let’s say 140,000 separate MinION reads, for instance…) … Continue reading

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Messing about with the MinION

Molecular phylogenetics – uncovering the history of evolution using signals in organisms’ genetic sequences – is a powerful science, the latest expression of the human desire to understand our common origins. But for all its achievements, I’d always felt something … Continue reading

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